Federal Medical & Biological Agency
Research Institute of Physical-Chemical Medicine
Website of Extracellular Vesicles Research Group

Centrifugation Parameters Calculator

1. Select your rotor

 

SW - Rotor

 
Rmin mm

Rmax mm

 

 

 

FA - Rotor

 Rmin mm
 Rmax mm
 Angle  o
Tube Diameter mm

 

List of standart rotors

 

 

 

 

 



2. Properties of the centrifugation medium

 

Vesicles density

g/cm3

Medium density

g/cm3

Medium viscosity

cP

 



3. Calculations

          Two of three parameters (RCF/RPM, centrifugation time, “cut-off-size” of completely sedimented vesicles) have to be fixed, the third one will be calculated upon pressing the button “calculate”.  The proportion of pelleted vesicles in the specified population of interest is calculated automatically.

Centrifugation protocol:

 

Rotation speed

relative centrifugal force (RCF)
revolutions per minute (RPM)

 

 

Centrifugation time

min

 

Vesicles sedimentation profile:

 

Complete sedimentation “cut-off “ size

nm

 

 

Vesicles diameter:

nm

nm

nm

nm

nm

Portion of pelleted vesicles:

-

-

-

-

-

©  Laboratory of Molecular Human Genetics, Research Institute of Physical-Chemical Medicine, Moscow, Russia.